Uses of DNA Technology

Uses of DNA Technology Introduction DNA typing was first used in Great Britain for law enforcement purposes in the mid- 1980s and has revolutionized forensic science and the ability of law enforcement to match perpetrators with crime scenes. It wasnt employed in the United States until 1987. DNA profiling has changed forensic science. DNA technology has given police and the courts a means of identifying the suspects of rapes and murders. Thousands of cases have been closed and innocent suspects freed with guilty ones punished because of the power of a silent biological witness at the crime scene. Today, the Federal Bureau of Investigation performs the bulk of the forensic DNA typing for local and state law enforcement agencies. In criminal investigations, DNA from samples of hair, bodily fluids or skin at a crime scene is compared with those obtained from suspected suspects. (http://faculty.ncwc.edu) ‘DNA fingerprinting, or DNA typing (profiling) as it is now known, was first described in 1985 by an English genet icist named Alec Jeffreys. Dr Jeffreys found that certain regions of DNA contained DNA sequences that were repeated over and over again next to each other. He also discovered that the number of repeated sections present in a sample could differ from individual to individual. By developing a technique to examine the length variation of these DNA repeat sequences, Dr Jeffreys created the ability to perform human identity tests. (John Butler, 2005) Sir Alec John Jeffreys, was born 9 January 1950 at Oxford in Oxfordshire. He is a professor of genetics at theUniversity of Leicester, and he became an honorary freeman of the City of Leicester on 26 November 1992. (Leicester City Council, 1992) In 1994, he was knighted by her Majesty Queen Elizabeth II of the United Kingdom, for Services to Science and Technology. Jeffreys had a eureka moment in his lab in Leicester after looking at the X-ray film image of a DNA experiment at 9:05 am on Monday 10 September 1984, which unexpectedly showed both similarities and differences between the DNA of different members of his technicians family. (BBC Radio, December 9, 2007) Within about half an hour, he realized the possible scope of DNA fingerprinting, which uses variations in the genetic code to identify individuals. The method has become important in forensic science to assist police detective work, and it has also proved useful in resolving paternity and immigration disputes. (BBC Radio, December 9, 2007) The method can also be applied to non-human species, for example in wildlife population genetics studies. Before his methods were commercialized in 1987 his laboratory was the only center carrying out DNA fingerprinting in the world, and during this period of about two or three years it was very busy, receiving inquiries from all over the globe. (Ne ston, Giles February 2, 2004) The technique used by Dr Jeffreys to examine the VNTRs was called restriction fragment length polymorphism (RFLP) because it involved the use of a restriction enzyme to cut the regions of DNA surrounding the VNTRs. This RFLP method was first used to help in an English immigration case and shortly thereafter to solve a double homicide case. Since that time, human identity testing using DNA typing methods has been widespread. The past 25 years have seen tremendous growth in the use of DNA evidence in crime scene investigations as well as paternity testing. Today over 100 public forensic laboratories and several dozen private paternity testing laboratories conduct hundreds of thousands of DNA tests annually in the United States. In addition, most countries in Europe and Asia have forensic DNA programs. The number of laboratories around the world conducting DNA testing will continue to grow as the technique gains in popularity within the law enforcement com munity. (John Butler, 2005) How DNA Typing Is Done Only one-tenth of a single percent of DNA (about 3 million bases) differs from one person to the next. (Internet Source www.ornl.org, 2009) Scientists can use these variable regions to generate a DNA profile of an individual, using samples from blood, bone, hair, and other body tissues and products. In criminal cases, this generally involves obtaining samples from crime-scene evidence and a suspect, extracting the DNA, and analyzing it for the presence of a set of specific DNA regions (markers). Scientists find the markers in a DNA sample by designing small pieces of DNA (probes) that will each seek out and bind to a complementary DNA sequence in the sample. A series of probes bound to a DNA sample creates a distinctive pattern for an individual. Forensic scientists compare these DNA profiles to determine whether the suspects sample matches the evidence sample. A marker by itself usually is not unique to an individual; if, however, two DNA samples are alike at four or five regions, o dds are great that the samples are from the same person. If the sample profiles dont match, the person did not contribute the DNA at the crime scene. If the patterns match, the suspect may have contributed the evidence sample. While there is a chance that someone else has the same DNA profile for a particular probe set, the odds are exceedingly slim. Many judges consider this a matter for a jury to take into consideration along with other evidence in the case. (Internet Source www.nfstc.org, 2009) Experts point out that using DNA forensic technology is far superior to eyewitness accounts, where the odds for correct identification are about 50:50. The more probes used in DNA analysis, the greater the odds for a unique pattern and against a coincidental match, but each additional probe adds greatly to the time and expense of testing. Four to six probes are recommended. Testing with several more probes will become routine, observed John Hicks (Internet Source www.alabany.edu/nerfi, 200 9). He predicted that DNA chip technology will enable much more rapid, inexpensive analyses using many more probes and raising the odds against coincidental matches. Types of DNA Technologies Restriction Fragment Length Polymorphism RFLP is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFLPs can be used in paternity cases or criminal cases to determine the source of a DNA sample. RFLPs can be used determine the disease status of an individual. RFLPs can be used to measure recombination rates which can lead to a genetic map with the distance between RFLP loci measured in centiMorgans. (Internet Source www.bio.davidson.edu, 2009) Total DNA is first extracted from the microbial community and the16S rRNA gene  is amplified from samples using fluorescently-labeled forward and reverse primers. Next, the PCR product is purified and subjected to restriction enzyme digestion with enzymes that have 4 base pair recognition sites. This step generates fluorescently-labeled terminal restriction fragments. The digested products are then se parated and detected on an appropriate electrophoresis platform. For a given sample the terminal fragments will contain a fluorescent label at the 5 end and will therefore be detected. The output will be a series of peaks (fragments) of various sizes and heights that represents the profile of that sample. (Osborn, A. M., Moore, R.B. and Timmis, K.N., 2000) Polymerase chain reaction PCR is used to make millions of exact copies of DNA from a biological sample. DNA amplification with PCR allows DNA analysis on biological samples as small as a few skin cells. A polymerase is a naturally occurring enzyme, a biological macromolecule that catalyzes the formation and repair of DNA (and RNA). The technique was made possible by the discovery of Taq polymerase, the DNA polymerase that is used by the bacterium Thermus auquaticus that was discovered in hot springs. This DNA polymerase is stable at the high temperatures need to perform the amplification, whereas other DNA polymerases become denatured. Since this technique involves amplification of DNA, the most obvious application of the method is in the detection of minuscule amounts of specific DNAs. This is important in the detection of low level bacterial infections or rapid changes in transcription at the single cell level, as well as the detection of a specific individuals DNA in forensic sci ence. It can also be used in DNA sequencing, screening for genetic disorders, site specific mutation of DNA, or cloning or subcloning of cDNAs. (Internet Source www.plattsburgh.edu, 2009) Short tandem repeat STR technology is used to evaluate specific regions (loci) within nuclear DNA. Variability in STR regions can be used to distinguish one DNA profile from another. The Federal Bureau of Investigation (FBI) uses a standard set of 13 specific STR regions for CODIS. CODIS is a software program that operates local, state, and national databases of DNA profiles from convicted offenders, unsolved crime scene evidence, and missing persons. The odds that two individuals will have the same 13-loci DNA profile is about one in a billion. (Internet Source www.ornl.org, 2009) The Federal Bureau of Investigation (FBI) has chosen 13 specific STR loci to serve as the standard for CODIS. The purpose of establishing a core set of STR loci is to ensure that all forensic laboratories can establish uniform DNA databases and, more importantly, share valuable forensic information. If the forensic or convicted offender CODIS index is to be used in the investigative stages of unsolved cas es, DNA profiles must be generated by using STR technology and the specific 13 core STR loci selected by the FBI. (Internet Source www.dna.gov, 2009) Mitochondrial DNA analysis mtDNA can be used to examine the DNA from samples that cannot be analyzed by RFLP or STR. Nuclear DNA must be extracted from samples for use in RFLP, PCR, and STR; however, mtDNA analysis uses DNA extracted from another cellular organelle called a mitochondrion. (Internet Source www.fbi.gov, 2009) While older biological samples that lack nucleated cellular material, such as hair, bones, and teeth, cannot be analyzed with STR and RFLP, they can be analyzed with mtDNA. In the investigation of cases that have gone unsolved for many years, mtDNA is extremely valuable. (Internet Source www.dna.com, 2009) All mothers have the same mitochondrial DNA as their offspring. This is because the mitochondria of each new embryo come from the mothers egg cell. The fathers sperm contributes only nuclear DNA. Comparing the mtDNA profile of unidentified remains with the profile of a potential maternal relative can be an important technique in missing-person investigations. ( Melton, T. et. al., 2001) Y-Chromosome Analysis The Y chromosome is passed directly from father to son, so analysis of genetic markers on the Y chromosome is especially useful for tracing relationships among males or for analyzing biological evidence involving multiple male contributors. Y chromosome analysis is a useful technique for analyzing DNA that can be likened in one sense to studying male surnames. Think about the way that male surnames are passed down from one generation to another and continue on through sons. This mechanism is a simplistic representation of Y chromosomes. A son inherits a Y chromosome from his biological father and he also inherits an X chromosome from his biological mother. Conversely, a female would inherit an X chromosome from her biological mother and an X chromosome from her biological father. (Internet Source www.esploredna.co.uk, 2009) As such, when scientists study Y chromosomes, they are studying these chromosomes as they are inherited over time through males in a famili al line. This type of DNA analysis has important ramifications for scientists wishing to investigate the familial ties between male members. (Internet Source www.ncbi.nlm.nih.gov, 2009) Conclusion No field has benefited more from the tools of molecular biology than forensic science. DNA technology affords the forensic scientist the ability to eliminate individuals who have been falsely associated with a biological sample and to reduce the number of potential contributors to a few (if not one) individuals. Inculpations are strong evidence regarding the source of the biological sample. Today, some wrongly convicted people have been exonerated because of DNA evidence. Moreover, in casework, individuals are excluded routinely. Since the inception of forensic DNA profiling, there has been a debate in the legal setting regarding admissibility on the methods and the practices of computing DNA profile frequencies. While the scientific basis of DNA typing were sound, both the methodology and the statistical interpretations were aggressively challenged in court. The methods challenge focused on reliability and validity testing. The statistics debate focused on the reliability of the ass umption of independence for applying the product rule to derive estimates of DNA profile frequencies. References Butler, John â€Å"Forensic DNA Typing: Biology, Technology, and Genetics of STR Markers† 2nd Edition 2005 Elsevier Science Desert Island Discs, â€Å"Desert Island Discs with Alec Jeffreys† BBC Radio 4 December 9, 2007 Leicester City Council â€Å"List of persons upon whom the honorary freedom of the city has been conferred† http://www.leicester.gov.uk/aboutleicester/lordmayorcivic/freeman/honorary-freemen/list-of-freemen Retrieved December 10, 2009 Newton Giles, â€Å"Discovering DNA fingerprinting: Sir Alec Jeffreys describes its development†. Wellcome Trust. http://genome.wellcome.ac.uk.doc Retrieved December 10, 2009 Osborn, A.M., Moore, R.B. and Timmis, K.N. (2000). An evaluation of terminal-restriction fragment lengty polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics. Environmental Microbiology 2(1): 39-50. Journal of Forensic Science â€Å"Diversity and Heterogeneity in Mitochondrial DNA of North American Populations.† January 2001; 46 (1):46-52. Melton T. et al Internet Source www.ornl.org 2009 Internet Source www.plattsburgh.edu 2009 Internet Source www.fbi.gov 2009 Internet Source www.dna.com, 2009

Opinion on Internet Privacy Essay

When thinking of internet security, my expectations change depending on where I am accessing the internet from. When I am accessing the web from home I feel some sort of safety because I have lots of different protection on my home computers as well as a secured network site key that cannot be accessed by my neighbors or anyone else unless my password is given to them by me. Although I have all these things that does not mean that hackers cannot get into my computer system so I am still very careful as to which sites I enter information onto. At work, I expect them to have some of the best internet security because if ever there is a problem they will have so much more too loose than an average person. Most businesses have things such as financial records and information that should not be accessed by outsiders. With there being a lot of business and organizations that compete they should be careful to make sure their private information cannot be stolen or viewed by anyone other than the people that the information is intended for. In a public setting, I will use my computer but I do understand that when doing so some of my information may be seen or accessed by others. Because of this, I am very careful of the sites that I visit. I do not enter personal information like Credit Card numbers, social security numbers, addresses or any information that I would not want to be in the eyes or hands of a stranger or a computer hacker. In my opinion, I would say that is it not legal or ethical to use your neighbor’s internet signal without their permission. Being a person who pays for internet service, I think that it is unfair to have someone else use your internet service at their convenience and not have to pay anything at all. Also because it is still unclear what information may be able to be seen or accessed by others I would say it is also unsafe to do so.

Nursing Fundamentals High School Class Essay Samples Options

Nursing Fundamentals High School Class Essay Samples Options The Good, the Bad and Nursing Fundamentals High School Class Essay Samples Insurers aren't going to pay for what isn't documented. Nursing orders are instructions for the particular pursuits that will perform to assist the individual achieve the medical care goal. For more information regarding this training course, speak to the American Heart Association. Nursing Fundamentals High School Class Essay Samples Help! Whether you want it or not, donations are an essential part of any class reunion. Ordering one alumni item per guest would be a fantastic way to not only commemorate the reunion, but in addition offer each attendee something that enables them to celebrate their time in school. The program I attend is just for the junior and senior years of high school. Your home school district might even supply free on-line classes which will help you graduate early. For those who haven't already done so, you are going to want to identify what year your son or daughter will be graduating. Furthermore, you would believe everyone knows everyone because my school is so small but that isn't true. Begin with the regional high school graduating classes across the area, not merely a single high school. Don't be scared to create the incorrect thing the incorrect wayit's all excellent practice, and you're going to learn whyit was wrong and that usually means that the following things you build will be better. Be realistic as you are setting up goals. 1 approach to define civics education is to concentrate on the creation of knowledge, skills, and dispositions necessary to constructively take part in democracy. As stated by the ministry's outline, students will be asked to compare Bitcoin with fiat currencies, which will gradually lead to basic unde rstanding of Bitcoin, cryptocurrencies and their function in the standard financial world. The Meaning of Nursing Fundamentals High School Class Essay Samples A class ring doesn't need to be an immense expense or strain on the budget. Several online classes are free and you will not need to scrape off extra dollars for the expenses mentioned previously. How to Find Nursing Fundamentals High School Class Essay Samples The professor can assist you during office hours. It's also good to invite aged teachers and staff if they're readily available. In case the school provides high school classes, it must be accredited to supply high school classes. Many schools provide distance learning, direct education or internet classes. Also, check to determine if a new book includes a CD-ROM. Online high school classes are a great option for homeschoolers. Taking high school classes it is a good option for any non-traditional student. Obviously, you will want to especially concentrate on science and math. All the companies which provide class reunion websites provide templates with amazing navigation and colors to coincide. If you've dismissed the concept of buying a class ring because of cost, you might want to reconsider. A class ring is a particular part of jewelry which holds memories and beauty. CenterpiecesFlowers in school colors are a good choice for center displays. Mathematics is about reasoning about the intellectual legwork, the trial-and-error procedure, and teamwork it can take to arrive at a remedy to an intricate issue. On-line classes are a little different from the conventional on-campus practice. You may want to limit the variety of nominations from the ground. School seating is largely designed for the typical student and we know there's no normal student. The modern-day American school system doesn't encompass or define learning, it's a dominant subset of it. In front of the whole class.

What Are The Causes Poverty - 1745 Words

What are the causes of poverty? There are many causes of poverty. The countries with extreme poverty have a lack of access to not only the proper foods, but health services and education as well. These countries also show a lack of inclusion, as many of the world’s poorest people do not receive any representation in the economy or in politics. The lack of education and representation creates an issue of mobility. Poverty is one of the most controversial topics that the governments have to deal with. The purpose of this paper is to point out that the root cause of poverty is lack of education. And education is also a cure for poverty. The clear path forward is to realize that the premise that poor children can’t be taught is wrong and to give our city’s children a good academic, social, behavioral and physical education right now. The human potential currently going to waste could be turned into the economic engine one that would make our region the envy of the na tion and demonstrate a better route to prosperity than building stadiums and other physical investments. Lack of Education and Poverty is an Outbreak Cycle Most of humanity lives on just a few dollars a day. There are high levels of inequality whether in the wealthiest nations or the poorest in the world. The poorest people have less access to health, education and other services; they hardly discern what is right or wrong since learning about moral or science is somewhat luxury. Lack ofShow MoreRelatedWhat Causes The Causes Poverty? Essay1164 Words   |  5 PagesEssay1 What Causes Poverty? Everybody is wondering why people are suffering from this kind of global issue? It is a severe case that takes over the world and kills the victims whom suffer from it. We should first know that this situation can be enhanced during the time. Undoubtedly it is poverty which is a state of being extremely poor. Subsequently, it is one of the most unsolved issues today. It leads into a bad condition. So, there are three major points that causes this situation. First, theRead MoreWhat Is The Cause Of Poverty?959 Words   |  4 PagesWhat is the cause of Poverty? In this paper I will discuss the causes for those below the poverty line in America set by the census bureau and how we might begin fixing them and how they are already being fixed. I believe these issues are able to be helped but they need to be clarified and addressed properly. Currently in America there are 46.2 million people currently listed as impoverished according to the Census Bureau. As such first we need to look at the causes for poverty for nationally. InRead MoreWhat Are the Causes of Poverty in Philippine Economy?4902 Words   |  20 PagesWhat are the causes of poverty in Philippine economy? 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